| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Luo, Matthew Z (14)
|
NCWOW |
35.94 |
35.19 |
-0.75 |
| 2 |
Setzer, Sean T (12)
|
NCWOW |
41.29 |
35.62 |
-5.67 |
| 3 |
Anderson, Case M (13)
|
NCECA |
40.99 |
38.51 |
-2.48 |
| 4 |
Hawthorne, Maxwell S (10)
|
NCWOW |
41.38 |
38.75 |
-2.63 |
| 5 |
Johnson, Cameron M (12)
|
NCECA |
46.93 |
40.12 |
-6.81 |
| 6 |
Lin, Owen N (9)
|
NCECA |
36.62 |
43.35 |
6.73 |
| 7 |
Mihaly, Lysander S (8)
|
NCCAT |
45.63 |
46.96 |
1.33 |
| 8 |
Johnson, Samuel T (10)
|
NCECA |
52.65 |
49.09 |
-3.56 |
| 9 |
Gemelli, Dominick (9)
|
NCWOW |
51.31 |
59.68 |
8.37 |
| 10 |
Sterner, Samuel R (7)
|
NCWOW |
1:05.16 |
1:05.49 |
0.33 |
| 11 |
Stephenson, Lucas T (9)
|
NCECA |
|
1:10.80 |
- |
| 12 |
Seymore, Davin J (6)
|
NCECA |
|
2:03.41 |
- |
|
Stephenson, Jack T (13)
|
NCECA |
|
DQ |
- |
|
Stephenson, Nathaniel R (10)
|
NCECA |
|
DQ |
- |
|
Sudmeyer, Samuel S (11)
|
NCECA |
1:12.93 |
NS |
- |
|
Fairchild, Philman J (7)
|
NCWOW |
1:26.44 |
NS |
- |